The sedimentation constant of actin of smooth muscle (3,6 S) is higher than that of striated muscle (3,0 S). The effect of denaturant (urea) and reducing (guanidine and tyoglicolic acid) substances on the two proteins is different. Small differences are remarked in the possibility of polimerization and reaction with myosin. Immunological tests have been used to investigate the difference between the two proteins after purification by gel filtration.
Referenze Bibliografiche
[1] CARSTEN M. E. e KATZ A. M., «Bioch. Bioph. Acta», 90, 534 (1964).
[2] CARSTEN M. E., «Biochemistry», 4, 1049 (1965).
[3] HANSON J. e LOWY J., «J. Mol. Biol.», 6, 46 (1963).
[4] Cfr. specialmente: CIGADA M., CITTERIO P., ORLANDI A., RANZI S. e TOSI L., «Ist. Lomb. Rend. Sc.», 82, 351 (1949); RANZI S., «Monit. Zool. Ital.», Supplem., 66, 1 (1958); AMBROSOLI MOGNONI G., «Ist. Lomb. Rend. Sc.», B 96, 154 (1962); SAWADA H., «Accad. Naz. Lincei (Rend. Sc. fis.)», 39, 352 (1965).
[5] MOMMAERTS W. F. H. M., «J. Biol. Chem.», 198, 445 (1952).
[6] ADELSTEIN R. S., GODFREY J. E. e KIELLEY W. W., «Biochem. Bioph. Res. Commun.», 12, 34 (1963).
[7] SZENT-GYÖRGYI A., Chemistry of muscular contraction, «Academic Press Inc.», New York, 2nd edition (1951).
[8] MIHASHI K., «Arch. Bioch. Bioph.», 107, 441 (1964).
[9] NAGY B. e YENKS W., «Biochemistry», 1, 987 (1962).
[10] KRANS H. M., VAN EIJK H. G. e WESTENBRINK H. G. K., «Bioch. Bioph. Acta», 100, 193 (1965).
Con il contributo del Ministero per i Beni e le Attività Culturali