Sono state preparate vescicole di membrana plasmatica da epatociti di ratto secondo due differenti metodiche, per saggiarne l'idoneità come substrato per lo studio del trasporto degli aminoacidi, tappa fondamentale del metabolismo aminoacidico nel fegato. A tale scopo sono stati studiati i sistemi di trasporto dell'alanina e dell'istidina. Si è potuto così identificare una preparazione in grado di conservare la capacità di accumulare entrambi gli aminoacidi con modalità e specificità, sia riguardo al substrato che agli ioni cotrasportati, analoghe a quelle già osservate nell'epatocita isolato.
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Con il contributo del Ministero per i Beni e le Attività Culturali