Nelle cellule epiteliali di cistifellea di coniglio sono state saggiate l'attività della ATPasi $Na^{+}—K^{+}$, della ATPasi anionica, della saccarasi, leucinoaminopeptidasi e fosfatasi alcalina. Si è tentato inoltre l’isolamento dell’intero orletto a spazzola e di frammenti di membrane apicali, nelle quali si è ricercata la presenza dell’ATPasi anionica (che potrebbe essere alla base dei trasporti di $HCO^{-}_{3}$, $Cl^{—}$ e $H^{+}$ rilevati in cistifellea). Come risultato l’ATPasi $Na^{+}—K^{+}$ è stata proposta come marcatore delle membrane baso-laterali e la fosfatasi alcalina delle membrane plasmatiche soprattutto apicali di cistifellea. Non si è riusciti ad isolare l’orletto a spazzola intero, ma si è giunti ad un discreto isolamento di membrane plasmatiche apicali col metodo della precipitazione con $Ca^{2+}$. Non è stata rilevata alcuna attività dell’ATPasi-anionica di tipo mitocondriale in questo tipo di membrane.
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Con il contributo del Ministero per i Beni e le Attività Culturali