In this work the haemagglutinins of Periplaneta americana L. hemolymph have been characterized from the point of view of functionality, biochemistry, and localization. Three proteins, which are able to agglutinate rabbit erythrocytes, have been isolated and purified. They show different molecular weights (respectively 72000, 24000 and 22000 Daltons); one of them is certainly a lectin. Both cytoplasmic and membrane-bound localization of the three haemagglutinins has been pointed out by differential immunofluorescence in the hemocytes. Only the haemocytes, which show membrane-bound haemagglutinins, are responsible for cellular reactions (i.c.a.). The possible phylogenetical origin of the two groups of haemagglutinins is discussed.
Referenze Bibliografiche
[1] R.T. Acton, P.F. Weinhemer and W. Niebermeir (1973) - The carbohydrate composition of invertebrate hemagglutinin subunits isolated from the lobster Palinurus argus and the oyster Crassostrea virginica, «Comp. Biochem. Phisiol.», 44, 185.
[2] J.E. Stewart and B.M. Zwickar (1972) - Natural and induced bacterial activities in the hemolymph of lobster Homarus americanus product of hemocyte-plasma interaction, «Can. J. Microbiol.», 18, 1499.
[3] C.J. Tyson and C.R. Jenkin (1972) - Phagocytosis of bacteria in vitro by hemocytes from the crayfish Parachaeraps bicarinatus, «The Australian Journal of Experimental Biology and Medical Science», 52.
[4] J.J. Marchalonis, G.M. Edelman (1968) - Isolation and characterization of a hemagglutinin from Limulus polyphemus, «J. Mal. Biol.».
[5] G.R. Seaman and N.L. Robert (1968) - Immunological responses of male cockroaches to injection of Tetraymena pyriformis, «Science».
[6] S. Hammarstrom and E.A. Rabat (1969) - Purification and characterization of a blood-group A reactive hemagglutinin study of its combining site, «Biochemistry», 8, 2696.
[7] R.T. Acton and E.E. Evans (1968) - Bacteriophage clearance in the oyster Crassostrea virginica, «J. Bact.», 95, 1260.
[8] G.B. Pauley, G.A. Granger and S.M. Krassner (1971) - Characterization of a natura agglutinin present in the hemolymph of the California sea hare: Aplysia California, «J. Invert. Pathol.», 18, 267.
[9] G.A. Amirante e S. Ferrario (1976) - Studi sulle caratteristiche immunologiche del teleosteo Tinca tinca L. Separazione e purificazione dell'eteroagglutinina a mobilità albuminica, «Acc. Naz. Lincei», 9, 4, 505.
[10] G.A. Amirante (1976) - Production of heteroagglutinins in hemocytes of Leucophaea maderae Fabr., «Experientia», 32, 526.
[11] G.A. Amirante and F. Guidali Mazzalai (1978) - Synthesis and localization of hemaogglutinins in hemocytes of the cockroach Leucophaea maderae Fabr., «Develop. Comp. Imm.», 2, 4, 735.
[12] F. Guidali Mazzalai, P. Bergamo e G.A. Amirante (1978) - Studi sulla sintesi di emoagglutinine da parte di colture di emociti di Insetti Blattoidei (Nauphoeta cinerea Oliv. e Leucophaea maderae Fabr.), «Acc. Naz. Lincei», 65, 5, 338.
[13] A.P. Gupta (1979) - Arthropod hemocytes and phylogeny, «Artropod Phylogeny» - Van Nostrand Reinhold - New York, 669.
[14] Y. Carton (1974) - Parentés entre les hémagglutinines naturelles d’échinodermes et les chaînes des immunoglobulines de vertébrés, «Ann. Immunol.», 125, 731.
Con il contributo del Ministero per i Beni e le Attività Culturali