Balanus amphitrite unsegmented eggs demonstrate a cholinesterase activity which does not change significantly during cleavage. Between stage 5 (early gastrula) and stage 12 (unhatched nauplius) the enzyme activity increases gradually about 15 times; a further increase, at a lower rate, takes place before hatching. $10^{-5}M$ Eserine causes a cholinesterase inhibition decreasing from approximately 75% at the early stages to 45% in the unhatched nauplius. Conversely, DFP inhibits cholinesterase increasingly from 8% to 30% (Table II). No effects are obtained with iso-OMPA. The results are discussed with regard to the function and specificity of cholinesterases in embryos of Balanus.
Referenze Bibliografiche
BUZNIKOV G. A., CHUKADOVA I. V., BERDISHEVA L. V. e VYAZMINA N. M. (1968) - The role of neurohumours in early embryogenesis. II: Acetylcholine and catecholamine content in developing embryos of sea urchin, «J. Embr. Exp. Morphol.», 20, 119-128.
CRISP D. J. (1954) - The breeding of Balanus porcatus (Da Costa) in the Irish sea, «J. Mar. Biol. Ass. U.K.», 33, 473-496.
DREWS U. (1975) - Cholinesterase in embryonic development. G. Fischer Verlag. Stuttgart.
FALUGI C. e RAINERI M. (1977) - Colinesterasi nello sviluppo di Crostacei, «Boll. Zool.» (in pubblicazione).
FUTAMACHI K. J. (1972) - Acetylcholine: possible neuromuscular transmitter in Crustacea, «Science», 175, 1373-
GREEN J. (1971) - Crustaceans. In: Experimental embryology of marine and fresh-water Invertebrates, G. Reverberi Ed., pp. 312-362. North Holland Publ. Co..
GROOM T. T. (1894) - On the early development of Cirripedia, «Phil. Trans. Roy. Soc. London», B, 185, 119-232.
GUSTAFSON T. e TONEBY M. (1970) - On the role of serotonin and acetylcholine in sea urchin morphogenesis, «Exp. Cell Res.», 62, 102-117.
HORRIDGE G. A. (1965) - Arthrofioda. In: Structure and function in the nervous system of Invertebrates, Bullock T. H. and Horridge G. A. Eds., II, 801-1270, Freeman and Co..
MINGANTI A., FALUGI C. e RAINERI M. (1973) - Colinesterasi in embrioni di Cirripedi, «Acta Embryol. Exper.», 234.
PATEL B. e CRISP D. J. (1960) - Rates of development of the embryos of several species of Barnacles, «Physiol. Zool.», 33, 104-119.
RAPPAPORT F., FISCHEL J. e PINTO N. (1959) - An improved method for the estimation of cholinesterase in serum, «Clin. Chim. Acta», 4, 227.
SPIELHOLZ N. I. e VAN DER KLOOT W. G. (1973) - Localization and properties of the cholinesterase in crustacean muscle, «J. Cell Biol.», 59, 407-420.
TREHERNE J. E. (1966) - The neurochemistry of Arthropods. Cambridge Univ. Press.
WIERSMA C. A. G.. (1961) - The neuromuscular system. In: The physiology of Crustacea, II, 191-240, Waterman T. H. Ed., Acad. Press..