The $\beta$ chains of the two hemoglobins typical of the adult chicken show the same electrophoretic mobility. When analyzed with the fingerprint technique, the two $\beta$ chains show a similar peptide pattern, while the tryptic maps of the two $\alpha$ chains are very different. The electrophoretic characteristics of the tryptic peptides are in agreement with those obtained by analyzing the single chains by polyacrylamide gel electrophoresis in acetic acid-urea. These results are strongly supported by the electrophoretic data described in literature and by those concerning the SH group reactivity of the single chains. The hemoglobins of the adult chicken differ from those of the mammals; in fact, while in mammals both fetal and adult hemoglobins have identical $\alpha$ chains, in the chick there is a strong evidence for a very similar structure of the $\beta$ chains.
Referenze Bibliografiche
ANTONINI E. e BRUNORI M. (1971) - Hemoglobin and myoglobin in their reactions with ligands. North-Holland Company, Amsterdam, p. 253.
BARGELLESI A., CALLEGARINI C. e CONCONI F. (1969) - Heterogeneity and globin composition of adult chicken hemoglobin, «Experientia», 25, 137.
CIROTTO G. e GERACI G. (1974 a) - Exposed sulphydryl groups of chicken haemoglobins: globin localization on their reactivity, «J. Mol. Biol.», 84, 103.
CIROTTO C. e D'AMELIO V. (1974 b) - Patrimonio globinico delle cellule eritroidi di pollo adulto. Analisi cromatografica, «Boll. Soc. It. Biol. Sper.», in corso di stampa.
CLEGG J. B., NAUGHTON M. A. e WEATHERALL D. J. (1966) - Abnormal human haemoglobins. Separation and characterization of the $\alpha$ and $\beta$ chains by chromatography, and the determination of two new variants, Hb Chesapeake and Hb J (Bangkok), «J. Mol. Biol.», 19, 91.
D'AMELIO V. (1966) - The globins of adult and embryonic chick hemoglobin, «Biochim. Biophys. Acta», 127, 59.
HASHIMOTO K. e WILT F. H. (1966) - The heterogeneity of chicken hemoglobin, «Proc. Natl. Acad. Sci. U.S.», 56, 1477.
HUISMAN T. H. J., SCHILLHORN VAN VEEN J. M., DOZY A. M. e NECHTMAN C. M. (1964) - Studies on animal hemoglobins. II. The influence of inorganic phosphate on the physicochemical and physiological properties of the hemoglobin of the adult chicken, «Biochim. Biophys. Acta», 88, 352.
HUNT Y., HUNTER T. e MUNRO A. (1969) - Control of haemoglobin synthesis: rate of translation of the messenger RNA for the $\alpha$ and $\beta$ chains, «J. Mol. Biol.», 43, 123.
INGRAM V. M. (1958) - Abnormal human haemoglobins. I. The comparison of normal human and sickle-cell haemoglobins by "finger printing", «Biochim. Biophys. Acta», 28, 539.
MALIK N. e BERRIE A. (1972) - New stain fixative for proteins separated by gel isoelectric focusing based on Coomassie Brilliant Blue, «Anal. Biochem.», 49, 173.
MATSUDA G. e TAKEI H. (1963) - The studies on the structure of chicken haemoglobin. I. Chromatografic purification of chicken haemoglobin, «J. Biochem. Tokyo», 54, 156.
MATSUDA G., TAKEI H., CHAYU K. e SHIOZAWA T. (1971) - Amino acid composition of the soluble tryptic peptides and chymotryptic peptides from the $\alpha$ polypeptide chain of A II component of chicken hemoglobin, «Int. J. Protein Research», III, 339.
ROSSI-FANELLI A., ANTONINI E. e CAPUTO A. (1958) - Studies on the structure of haemoglobin. I. Physicochemical properties of human globin, «Biochim. Biophys. Acta», 30, 608.
STEGINK L. D., MEYER P. D. e CHALKLEY R. (1971) - Acrylamide gel electrophoresis of hemoglobin polypeptide chains, «Anal. Biochem.», 41, 351.
WALEY S. Q. e WATSON J. (1953) - The action of trypsin on polylysine, «Biochem. J.», 55, 328.
WILT F. H. (1967) - The control of embryonic hemoglobin synthesis, «Adv. Morphogenesis», 6, 89.
Con il contributo del Ministero per i Beni e le Attività Culturali