The localization of acetylcholinesterase (AChE) and pseudocholinesterase (PsChE) in adrenals of Rana esculenta and Bufo bufo was studied with Lewis and Shute method (1969). The activity of AChE in Rana is positive in the chromaffin cells and is localized in the nuclear envelope, in the cisternae of the rough endoplasmic reticulum and in some vesicles or cisternae of the vacuolar system, and in some chromaffin granules. The AChE reaction in Bufo chromaffin cells is less intense than in Rana's; it is positive in the vacuolar system; it rarely appears in the Golgi system and on the cell membrane for short lengths only. The nerve fibres located among the adrenal cells, both in Rana and Bufo, generally show a positive reaction to AChE located at axonal surfaces and in some vesicles and neurotubular structures within the axoplasm. The endings, whose morphologic aspect was cholinergic, generally react positively to AChE. No activity was found in Rana and Bufo adrenals when butyrylthiocholine was used as substrate.
Referenze Bibliografiche
BAYLISS B. J. e TODRICK A. (1956) - The use of a selective acetylcholinesterase inhibitor in the estimation of pseudocholinesterase activity in rat brain, «Biochem. J.», 62, 62.
BRZIN M., TENNYSON V. M. e DUFFY P. E. (1966) - Acetylcholinesterase in frog sympathetic and dorsal root ganglia, «J. Cell. Biol.», 31, 215.
CHACKO L. W. e CERF J. A. (1960) - Histochemical localization of cholinesterase in the amphibian spinal cord and alteration following ventral root section, «J. Anat.», 94, 74.
COUPLAND R. E. (1972) - The chromaffin system. In «Handbuch der experimentellen pharjmakologie», 33, 16.
EL-BADAWI A. e SCHENK E. A. (1967) - Histochemical methods for separate, consecutive and simultaneous demonstration of AChE and Norepinephrine in cryostat sections, «J. Histochem. Cytochem.», 15, 580.
FUKUDA T. e KOELLE G. B. (1959) - The cytological localization of intracellular neuronal acetylcholinesterase, «J. Biophysic, and Biochem. Cytol.», 5, 433.
KÀSA P. e CSILLIK B. (1968) - AChE synthesis in cholinergic neurons: electron histochemistry of enzyme translocation, «Histochemie», 12, 175.
KOELLE G. B. (1955) - The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons, «J. Pharmacol, and Exp. Therap.», 114, 167.
KOELLE G. B. e FOROGLOU-KERAMEOS C. (1965) - Electron microscopic localization of cholinesterase i?i a sympathetic ganglion by a gold-thiolacetic acid method, «Life Sciences», 4, 417.
KOELLE G. B. e FRIEDENWALD J. S. (1949) - A histochemical method for localizing cholinesterase activity, «Proc. Soc. Exp. Biol, and Med.», 70, 617.
KOENIG E. (1965) - Synthetic mechanisms in the axon. I. Local axonal synthesis of acethylcholinesterase, «J. Neurochem.», 12, 343.
LEWIS P. R. e SHUTE C. C. D. (1909) - An electron-microscopic study of cholinesterase distribution in the rat adrenal medulla, «J. Microscopy», 89, 181.
MANELLI H., MASTROLIA L. e GALLO V. P. (1973) - Attività acetilcolinesterasica e pseudocolinesterasiche nelle surrenali degli embrioni e degli adulti di pollo, «Riv. di Biol.», in corso di stampa.
MASTROLIA L. e MANELLI H. (1972) - Sulla presenza di alcune esterasi nelle surrenali di pollo, «Acta Embr. Exp.», 264.
PEARSE E. A. G. (1960) - Histochemistry theoretical and applied. 2nd Ed. Churchill. London.
PIEZZI R. S. (1966) - Two types of synapses in the chromaffin tissue of the toad's adrenal, «Acta Physiol. Latinoam.», 16, 282.
PIEZZI R. S. (1967) - Chromaffin tissue in the adrenalgland of the toad, Bufo arenarum Hensel, «Gen. Comp. End.», 9, 143.
ROSENBLUTH J. M. D. (1962) - Subsurface cisterns and their relationship to the neuronalplasma membrane, «J. Cell. Biol.», 13, 405.
SHEN S. C., GREENFIELD P. e BELL E. J. (1955) - The distribution of cholinesterase in the frog brain, «J. Comp. Neurol.», 102, 717.
Con il contributo del Ministero per i Beni e le Attività Culturali