The effects of the addition of fetal calf serum are described on the self-aggregation of embryonic locust cells obtained and seeded in culture according to the techniques already described (this «Rendiconti» 54, 795-802 (1973)). The main effect of the fetal calf serum, tested at different concentration, is the inhibition of the adhesion of the cells to the bottom of the culture dish; this effect is higher with the increase of serum concentration. In tissue culture media with 10 percent of fetal calf serum aggregates are easily formed and all are floating on the medium. The shape and the size of the aggregates obtained both of preblastokinetic and postblastokinetic embryos, in different media are more similar than the aggregates obtained without serum. The fetal calf serum aids the initial aggregation but, at the concentration of 10 per cent, the aggregates also at 24 h begin to disaggregate.
Referenze Bibliografiche
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Con il contributo del Ministero per i Beni e le Attività Culturali