Since few histochemical investigations draw attention to the presence of acetylcholinesterase (AChE) in the neurons of brain cortex and retina in tissue culture, it seemed interesting to study the distribution of this enzyme activity in the nerve cells grown in organotipic and tissue cultures of cerebellum. Cerebellar tissue from 14 and 16 day chick embryos and from adult animals was cultivated until 1 month. The methods by Koelle and by Karnowsky and Roots were followed for the localisation of AChEase activity. Our results suggested that the Purkinije cells grown in cultures have different histochemical characters from those observed in vivo. The significance of this enzyme distribution is discussed briefly.
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Con il contributo del Ministero per i Beni e le Attività Culturali