Stefanelli, Alberto:
L'effetto biologico delle elevate pressioni idrostatiche. Realizzazione di alcuni apparecchi per l'osservazione diretta del materiale
Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti Serie 8 52 (1972), fasc. n.4, p. 569-572, (Italian)
pdf (660 Kb), djvu (720 Kb).
Sunto
Experimental data of Marsland and Pease on the inhibitory effects of hydrostatic pressure (about 300-400 atm.) on cell division, as observed in segmenting eggs of a number of animal species, prompted me to investigate the process of embryonic morphogenesis in which cell division is brought about along with cell degeneration and movements, by means of this technique. To this aim I devised three pressurized chambers: two of them allow for direct observation of specimens, one being suitable for pressure values up to 600 atm., the other for values up to 1500 atm., while in the third chamber it is possible to fix specimens under pressure, either by means of a fixative, or by freezing them.
Referenze Bibliografiche
[1] FLÜGEL H. e SCHLIEPER C., The effect of pressure on marine invertebrates and fishes. High pressure effects on cellular processes, «Cell. Biol. Monografs», Ac. Press. (1970).
[2] FREUNDLICH H., Some recent work on gels, «J. Phys. Chem.», 41, 901-320 (1927).
[3] JOHNSON F. H., EYRING H. e POLISSAR M. J., The kinetic basis of molecular biology. Wiley, N.Y. (1954).
[4] MARSLAND D., Pressure-temperature studies on the mechanisms of cell division. In «High pressure effects on cellular processes», «Cell. Biol. Monografs», Ac. Press (1970).
[5] LANDAU J. V. e THIBODEAN L., The micromorphology of Amoeba proteus during pressure induced changes in the sol-gel cycle, «Exp. Cell Res.», 27, 591-594 (1962).
Con il contributo del Ministero per i Beni e le Attività Culturali