The ultrastructural characteristics of the skin of Triturus cristatus Laur. are described. The mucopolysaccaridic content in the cytoplasmic granules, present in all epidermal cells, was analyzed by means of “staining” with PTA. The presence of tyrosinase activity was evidenced by Dopa incubation in the same kind of cells; the intensity of the reaction, however, is different in the different cells and in the structures where this reaction appears.
Referenze Bibliografiche
BANI G., Osservazioni sulla ultrastruttura della etite di Bufo bufo (L.) e modificazioni a livello dell'epidermide, in relazione a differenti condizioni ambientali, «Monitore Zool. It.», 74, 93-112 (1966).
BRODY J., The keratinization of epidermal cells of normal Guinea Pig skin as revealed by electron microscopy, «J. Ultrastructure Res.», 2, 482-511 (1959).
CAMATINI M. e LANZAVECCHIA G., Osservazioni sull'ultrastruttura dell'epidermide in embrioni e larve di Rana esculenta L., «Accademia Naz. dei XL», 15, 1-18 (1965).
CARDELL R. R., BADENHAUSEN S. e PORTER K. R., Intestinal triglyceride absorption in the rat., «J. Cell. Biol.», 34, 123-155 (1967).
CHARLES A. e INGRAM J. T., Electron microscope observations of the melanocyte of the human epidermis, «J. Biophys. Biochem. Cytol.», 6, 41-44 (1959).
CLARK W. H. Jr. e HIBBS R. G., Electron microscope studies of the human epidermis. The clear cell of Masson (dendritic cell or melanocyte), «J. Biophys. Biochem. Cytol.», 4, 679-684 (1958).
FARQUHAR M. G. e PALADE G. E., Cell junctions in Amphibian skin, «J. Cell Biol.», 26, 263-291 (1965).
KELLY D. E., Fine structure of desmosomes, hemidesmosomes, and an adepidermal globular layer in developing newt epidermis, «J. Cell. Biol.», 28, 51-72 (1966).
MARINOZZI V., Phosphotungstic Acid (PTA) as a stain for polysaccharydes and glycoproteins in electron microscopy. «Electron Microscopy», Proc. 4th European Regional Conference on Electron Micr., Roma, Tipografia Poliglotta Vaticana, 55 (1968).
MILLONIG G., Further observations on a phosphate buffer for osmium solutions in fixation, in «Electron Microscopy», «Proc. 5th Inter. Congress for Electron Micr., Philadelphia, Academic Press ed., New York, 2, P-8 (1962).
MISHIMA Y., LOUD A. V. e SCHAUB F. F. Jr., Electron microscopy of premelanin, «J. Invest. Dermatol.», 39, 55-62 (1962).
PARAKKAL P. F. e MATOLTSY A. G., A study of the fine structure of the epidermis of Rana Pipiens, «J. Gell Biol.», 20, 85-94 (1964).
PEROTTI M. L., Gytochemical techniques in electron microscopic studies of melanin synthesis, «Atti 5th Congress It. Microscopia Electr.», 37-40 (1965).
PILLAI P. A., Electron microscopic studies on the epidermis of newt with an enquiry into the problem of induced neoplasia, «Protoplasma», 55, 10-62 (1962).
SALPETER M. M. e SINGER M., The fine structure of the adepidermal reticulum in the basal membrane of the skin of the newt Triturus, «J. Biophys. Biochem. Cytol.», 6, 35-40 (1959).
SALPETER M. M. e SINGER M., Differentiation of the submicroscopic adepidermal membrane during limb regeneration in adidt Triturus, including a note on the use of the term basement membrane, «Anat. Rec.», 136, 27-40 (1960).
SEIJI M., Subcellular particles and melanin formation in melanocytes, in «The Pigmentary System», Montagna ed., Pergamon Press, 8, 189-222 (1966).
REYNOLDS E. S., The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, «J. Cell Biol.», 17, 208-212 (1963).
ZAVANELLA T., Comunicazione personale.
Con il contributo del Ministero per i Beni e le Attività Culturali