Purine and pyrimidine bases and related nucleosides in seeds and seedlings of Phaseolus vulgaris. The common purine and pyrimidine bases and related nucleosides occurring in 1200 quiescent embryos of Phaseolus vulgaris have been isolated and identified using a ion-exchange column (Dowex 2X) with ammonium formate solutions, paper chromatography and absorbtion spectra at various pH. The results have been compared with those obtained in previous studies [1] [2] [3]. All the naturally occurring bases in the nucleic acids except Tymine were found in seedlings. No evidence for the presence of Tymine and Cytosine in quiescent cotyledons and Tymine, Cytosine and Guanine in quiescent embryos has been obtained. All the common ribonucleosides are present both in quiescent and in germinating seeds. Deoxy-ribonucleosides were not found in quiescent seeds but they were always present in germinating ones.
Referenze Bibliografiche
[1] M. BELLANDO, I precursori non fosforilati degli A. N. nei cotiledoni di Phaseolus vulgaris allo stato quiescente, «Acc. Naz. dei Lincei Rend. Sc. Fis. mat. nat.», Ser. VIII, vol. XXXIV, 5 (1963).
[2] M. BELLANDO e N. FIUSSELLO, I precursori non fosforilati degli A. N. negli embrioni germinatiti di Phaseolus vulgaris, «Acc. Naz. dei Lincei Rend. Sc. Fis. Mat. Nat.», Ser. VIII, vol. XL, 1 (1966).
[3] M. BELLANDO e N. FIUSSELLO, I precursori non fosforilati degli A. N. nei cotiledoni germinanti di Phaseolus vulgaris, «Acc. Naz. dei Lincei Rend. Sc. Fis. Mat. Nat.», Ser. VIII, vol. XLI, 5 (1966).
[4] W. E. COHN, «J. Am. Chem. Soc.», 72-1471 (1950).
[5] E. CHARGAFF e J. N. DAVIDSON, The nucleic acids, - N. Y. (1955).
[6] G. DUPONT e A. KIRRMAN e Altri, «Chromatogr. en Chim. org. et biol.», Paris 1960.
[7] A. LARSSON e P. REICHARD, Progress in Nucleic Acid Research and Molecular Biology, «Academic Press - N. Y.», 7, 303 (1967).
Con il contributo del Ministero per i Beni e le Attività Culturali