A histochemical study has been made of the composition of the larval tunic of the ascidian Ciona intestinalis. The tunic was found to contain (a) proteins, possibly stabilized by SH-group and by quinone groups, and (b) neutral and acid polysaccharides, with sulfate groups. Proteins and polysaccharides are likely to be combined into glycoproteins and mucopolisaccharides of different kinds. No evidence of the presence of cellulose in the tunic has been obtained.
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