Bellando, Mario and Fiussello, Nevio:
I precursori non fosforilati degli acidi nucleici nei cotiledoni germinanti di Phaseolus vulgaris
Atti della Accademia Nazionale dei Lincei. Classe di Scienze Fisiche, Matematiche e Naturali. Rendiconti Serie 8 41 (1966), fasc. n.5, p. 365-367, (Italian)
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Sunto
The Authors have isolated and identified the purine and pyrimidine bases and related nucleosides occurring in the free state in cotyledons of etiolated dwarf bean seedling. 180 cotyledons from young etiolated plants (23-26 cm in length) were collected, washed and homogenized at 4°C. The resulting slurry was dyalized against fresh water and the proteins and polysaccharides-free solution concentrated and analyzed in an ion-exchange column (Dowex 2 X, formate). The elution was achieved with ammonium formate solutions with discontinuously increasing ionic strength and pH. The fractions were collected, dried in vacuo and the solid ammonium formate sublimed. The costiti!ents were purified chromatographically and identified by their U.V. absorbtion spectra at various pH. The relative abundance of the components was also, when possible, roughly evaluated.
Referenze Bibliografiche
[1] M. BELLANDO, I precursori non fosforilati degli A.N. nei cotil. di Ph. vulgaris allo stato quiescente, «Acc. Naz. dei Lincei», Rend. Sc. fis. mat. nat., ser. VIII, vol. XXXIV, 5 (1963).
[2]
M. BELLANDO e
N. FIUSSELLO,
I precursori non fosforilati degli A.N. negli embrioni germinanti di Ph. vulgaris, «
Acc. Naz. dei Lincei», Rend. Sc. fis. mat. nat., ser. VIII, vol.
XL, 1 (
1966). |
fulltext bdim[3] W. E. COHN, «J. Am. Chem. Soc.», 72, 1471 sgg. (1950).
[4] E. CHARGAFF e J. N. DAVIDSON, The nucleic acids, N. Y. (1955).
[5] G. DUPONT e A. KIRRMAN et al., Chromatogr. en Ch. org. et biol., Paris (1960).
[6] E. STAHL., Dunnschichtckromatographie, Berlin (1962).
[7] H. M. MERSCHENSON, U.V. and visible absorption spectra, N. Y. (1956).
Con il contributo del Ministero per i Beni e le Attività Culturali